Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10825886 | Methods | 2013 | 6 Pages |
Abstract
S-nitrosylation of protein cysteine residues is known to be an important mechanism for nitric oxide signaling. However, the detection of protein S-nitrosylation is still challenging due to technical limitations of current methods. This chapter provides a brief review on recent developments of methods, which directly target S-nitroso moieties for detection. We also describe in detail the protocol of an organophosphine-based biotin labeling of protein S-nitroso moieties.
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Authors
Nelmi O. Devarie-Baez, Dehui Zhang, Sheng Li, A. Richard Whorton, Ming Xian,