Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10825913 | Methods | 2013 | 9 Pages |
Abstract
⺠Microfluidic arrays enable analysis of 96 qPCR assays on 1440 single cells. ⺠Detailed methods on obtaining qPCR data and performing preliminary data processing. ⺠Data from sufficient cells to address noise inherent in single-cell transcription. ⺠Methods used for conventional qPCR do not necessarily apply to single-cell qPCR.
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Authors
Kenneth J. Livak, Quin F. Wills, Alex J. Tipping, Krishnalekha Datta, Rowena Mittal, Andrew J. Goldson, Darren W. Sexton, Chris C. Holmes,