| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10826043 | Methods | 2013 | 14 Pages |
Abstract
⺠The neurotensin receptor NTS1 was reconstituted into nanodiscs. ⺠We characterized these by light scattering and analytical ultracentrifugation. ⺠These biophysical methods do not require any sample labeling or modification. ⺠In this manner we confirmed the nanodisc receptor and lipid stoichiometry. ⺠The influence of lipid charge on the signaling properties of NTS1 was investigated.
Keywords
MSP2xYTNTS1TrxAPOPGNanodiscH10PoPCGPCRIMACDLSE. coliDDMMBPCHSnuclear magnetic resonance1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol)1-Palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholineAUCHPLC/MS/MSn-Dodecyl-β-D-maltosideanalytical ultracentrifugationPOPsEscherichia coliTevNMRSedimentation equilibriumPhase transition temperatureLipid bilayerSedimentation velocityneurotensinTobacco etch virusDynamic Light Scatteringmembrane scaffold proteinMembrane proteinmaltose-binding proteinCHAPSimmobilized metal affinity chromatographyneurotensin receptor 1G protein-coupled receptor
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Authors
Sayaka Inagaki, Rodolfo Ghirlando, Reinhard Grisshammer,