Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10826104 | Methods | 2011 | 12 Pages |
Abstract
Transcription factors (TFs) play a central role in the development of multicellular organisms. The sequential actions of critical TFs direct cells to adopt defined differentiation pathways leading to functional, fully differentiated tissues. Here, we describe a generic experimental pipeline that integrates biochemistry, genetics and next generation sequencing with bioinformatics to characterize TF complexes composition, function and target genes at a genome-wide scale. We show an application of this experimental pipeline which aims to unravel the molecular events taking place during hematopoietic cell differentiation.
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Biochemistry
Authors
Eric Soler, Charlotte Andrieu-Soler, Ernie de Boer, Jan Christian Bryne, Supat Thongjuea, Erikjan Rijkers, Jeroen Demmers, Wilfred van IJcken, Frank Grosveld,