| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10826136 | Methods | 2012 | 13 Pages |
Abstract
⺠NMR is used to directly monitor activation state of GTPase proteins in real time. ⺠Application to intrinsic nucleotide hydrolysis/exchange as well as GAP/GEF assays. ⺠Unambiguous readout enables assays of GAP/GEF activities in mammalian cell lysates. ⺠Small GTPases Ras, RhoA, and Rheb are presented examples. ⺠Activities of TSC2, p120 RasGAP, Sos, PDZ-RhoGEF and Lfc/GEF-H1 were assayed.
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Authors
Christopher B. Marshall, David Meiri, Matthew J. Smith, Mohammad T. Mazhab-Jafari, Geneviève M.C. Gasmi-Seabrook, Robert Rottapel, Vuk Stambolic, Mitsuhiko Ikura,