Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10826151 | Methods | 2012 | 9 Pages |
Abstract
Two-dimensional (2D) agarose gel electrophoresis is one of the most powerful methods to analyze the mass and shape of replication intermediates. It is often use to map replication origins but it is also useful to characterize termination of replication, replication fork barriers and even replication fork reversal. Here, we present protocols, figures and movies with a thorough description of different modes of replication for linear DNA fragments and the corresponding patterns they generate in 2D gels.
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Authors
Jorge B. Schvartzman, MarÃa-Luisa MartÃnez-Robles, Virginia López, Pablo Hernández, Dora B. Krimer,