| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10826156 | Methods | 2012 | 8 Pages |
Abstract
⺠Detailed protocols for purification of various assemblies of Mcm complexes using an insect cell expression system are described. ⺠A highly efficient expression system in human 293T cells for large-sized proteins and complexes is described. ⺠Detailed description of transfection with in-house reagents is provided. ⺠Procedures for purification of various replication factors with this system are described. ⺠Other uses of this expression system are described.
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Authors
Shuji Uno, Zhiying You, Hisao Masai,