| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10826225 | Methods | 2011 | 9 Pages |
Abstract
⺠A suite of N-terminal hexa-histidine tagged fusion protein vectors is presented. ⺠9 fusion proteins can be made from one PCR product using Infusion⢠cloning. ⺠The effect of fusions on the solubility of 20 proteins in E coli was tested in 2 media. ⺠The SUMO tag was the most effective at solubilising targets. ⺠Auto-induction media gave more high level expressers than IPTG induction.
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Authors
Louise E. Bird,