Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10826667 | Methods | 2005 | 6 Pages |
Abstract
One current challenge facing point-of-care cancer detection is that existing methods make it difficult, time consuming and too costly to (1) collect relevant cell types directly from a patient sample, such as blood and (2) rapidly assay those cell types to determine the presence or absence of a particular type of cancer. We present a proof of principle method for an integrated, sample-to-result, point-of-care detection device that employs microfluidics technology, accepted assays, and a silica membrane for total RNA purification on a disposable, credit card sized laboratory-on-card ('lab card”) device in which results are obtained in minutes. Both yield and quality of on-card purified total RNA, as determined by both LightCycler and standard reverse transcriptase amplification of G6PDH and BCR-ABL transcripts, were found to be better than or equal to accepted standard purification methods.
Keywords
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Authors
M. Kokoris, M. Nabavi, C. Lancaster, J. Clemmens, P. Maloney, J. Capadanno, J. Gerdes, C.F. Battrell,