| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10833115 | Molecular Genetics and Metabolism | 2013 | 9 Pages |
Abstract
⺠Transmembrane proteins form insoluble aggregates with traditional expression systems. ⺠Baculovirus (BV) infection of Spodoptera cells (Sf9) causes release of microvesicles. ⺠BV containing CTNS or SLC17A5 sequences yield Sf9 vesicles containing cystinosin or sialin ⺠Such vesicles cause cystine or sialic acid depletion of cystinotic or ISSD fibroblasts. ⺠This method may permit production of other functional transmembrane proteins.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Jess Thoene, Thomas Goss, Marc Witcher, Jodi Mullet, Francisca N'Kuli, Patrick Van Der Smissen, Pierre Courtoy, Si Houn Hahn,