Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10833544 | Molecular Genetics and Metabolism | 2012 | 6 Pages |
Abstract
⺠Tâcell receptor excision circle (TREC) count by PCR shows thymic output of T cells. ⺠An EBV Bâcell line was made with a single copy of the TREC sequence. ⺠Graded TREC-EBV cell numbers added to T-depleted blood constituted calibrators. ⺠TREC copy number was proportional to the number of TREC-EBV cells. ⺠TREC-EBV cells are as a universal cellular calibrator for TREC assays.
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Authors
Divya Punwani, Diana Gonzalez-Espinosa, Anne Marie Comeau, Amalia Dutra, Evgenia Pak, Jennifer Puck,