Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10834837 | Molecular Genetics and Metabolism | 2005 | 5 Pages |
Abstract
Members of the nuclear receptor superfamily are ligand dependent transcription factors and many of the receptors are difficult to produce in their functional form. Here, we describe a method for obtaining functional nuclear receptor ligand binding domain proteins from bacterial expressed inclusion bodies by high hydrostatic pressure induced refolding. High pressure refolding successfully reconstituted activity from several insoluble nuclear receptor proteins and represents a valuable tool for both functional and structural investigation of proteins or fragments thereof that might otherwise remain insoluble.
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Authors
Brigitte E. Schoner, Kelli S. Bramlett, Haihong Guo, Thomas P. Burris,