Nesfatin-130-59 but not the N- and C-terminal fragments, nesfatin-11-29 and nesfatin-160-82 injected intracerebroventricularly decreases dark phase food intake by increasing inter-meal intervals in mice

► Nesfatin-11-82 injected icv reduces dark phase feeding in mice. ► The mid-fragment nesfatin-130-59 dose-dependently reduces 4-h food intake. ► The reduction goes along with reduced meal frequency and longer inter-meal interval. ► Neither nesfatin-11-29 nor nesfatin-160-82 reduce dark phase food intake. ► Nesfatin-130-59 is the active core of nesfatin-11-82.