An integrin-binding N-terminal peptide region of TIMP-2 retains potent angio-inhibitory and anti-tumorigenic activity in vivo

► The α3β1 binding domain is localized to the N-terminal domain of the TIMP-2. ► This integrin binding domain encompasses residues Ile43-Ala66, or B-C loop. ► Synthetic peptides containing sequences from this region suppress endothelial growth. ► Peptides from this region are anti-angiogenic and anti-tumorigenic in vivo. ► TIMP-2 binds to the endothelial cell surface through two distinct loops.