| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10835991 | Peptides | 2011 | 10 Pages |
Abstract
⺠We describe a multi-parameter high-content analysis protocol for assessing peptide toxicity on epithelial cells. ⺠Selected deletion of amino acids of the bee venom, melittin, reduces its cytotoxicity. ⺠Loss of cytotoxicity was correlated with loss of epithelial permeation enhancement but not a reduction in antimicrobial activity.
Keywords
MBHANMPDIEAMMPHBTUBOCFMOCt-butoxycarbonylHOBtFCCPTFA4-methylbenzhydrylamineN,N′-diisopropylethylaminet-butylN-MethylpyrrolidonePMPTRTcarbonyl cyanide p-trifluoromethoxy-phenylhydrazone9-fluorenylmethoxycarbonylN-hydroxybenzotriazoleMTT assayTrifluoroacetic acidt-Butritylcell numberCytotoxicity screeningMelittinNuclear AreaPlasma membrane permeabilityMitochondrial membrane potentialAntimicrobial peptideIntracellular calcium
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Authors
Edwin G. Walsh, Sam Maher, Marc Devocelle, Peter J. O'Brien, Alan W. Baird, David J. Brayden,