Serotonergic and dopaminergic distinctions in the behavioral pharmacology of (±)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD)

► The pharmacology of DOI and LSD were compared in vivo and in vitro using rabbits. ► Both DOI and LSD elicited head bobs through activation of 5-HT2A and D1 receptors. ► Both DOI and LSD bound 5-HT2A receptors, but only LSD bound D1 receptors. ► LSD and ritanserin pseudo-irreversibly bound frontocortical 5-HT2A receptors. ► DOI and ketanserin reversibly bound frontocortical 5-HT2A receptors.