The extracts of Fructus Akebiae, a preparation containing 90% of the active ingredient hederagenin: Serotonin, norepinephrine and dopamine reuptake inhibitor

► We found that that FAE displayed marked affinity to rat and cloned human monoamine transporters. ► FAE was found to significantly inhibit all three monoamine transporters. ► In this study we found that FAE showed no significant affinity to a variety array of receptors tested from CNS. ► The administration of FAE significantly increased the levels of NE, 5-HT, and DA in the prefrontal cortex of rats.