| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10838287 | Pharmacology Biochemistry and Behavior | 2011 | 11 Pages |
Abstract
⺠Pretreatment with mAb GNC92H2 IgG (Kd = 2 nM) prevents cocaine-induced lethality. ⺠Immunization with the IgG, F(abâ²)2, or Fab after cocaine (i.p.) reversed overdose. ⺠Only GNC92H2 F(abâ²)2 and Fab attenuated other symptoms of severe toxicity. ⺠The Fab pharmacokinetic profile most closely aligns to that of cocaine in mice. ⺠mAb GNC92H2 Fab represents a superior antidote for acute cocaine toxicity.
Keywords
Fisher's Protected Least Significant DifferenceVH and VLNIDAmAbIgGi.v.CSSVDSSscFvPCPt1/2Monoclonal antibodyPassive immunizationImmunopharmacotherapyimmunoglobulin Ganalysis of varianceANOVAELISAEnzyme-linked immunosorbent assayvolume of distribution at steady stateDissociation constantIntravenousCNScentral nervous systemFabPhencyclidineSingle-chain antibody fragmentnational institute on drug abusedrug overdoseMiceelimination half-lifeVaccineCocaine
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Authors
Jennifer B. Treweek, Amanda J. Roberts, Kim D. Janda,