| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843178 | Protein Expression and Purification | 2013 | 7 Pages |
Abstract
⺠Split-type ArcTGT was isolated from Methanosarcina acetivorans as a heterotetrameric complex. ⺠Recombinant ArcTGT was co-overexpressed by the two split peptides in Escherichia coli cells. ⺠One peptide alone did not result in expression products in soluble fractions. ⺠The kinetic parameters of the recombinant and native ArcTGTs were closely similar. ⺠Recombinant ArcTGT has potential to produce a possible intermediate, preQ0-tRNA.
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Authors
Yuichiro Nomura, Yumiko Onda, Satoshi Ohno, Hiroki Taniguchi, Kaori Ando, Natsuhisa Oka, Kazuya Nishikawa, Takashi Yokogawa,