Article ID Journal Published Year Pages File Type
10843185 Protein Expression and Purification 2013 6 Pages PDF
Abstract
► We report a new laccase from the filamentus fungus Podospora anserina. ► We overexpressed it in Pichia pastoris with a 65% yield and specific activity of 644 U mg−1. ► The new enzyme displays a half life > 400 min at 37 °C. ► It also displays a good stability at pH 7 and in presence of chloride.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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Purification and characterization of a new laccase from the filamentous fungus Podospora anserina
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