Article ID Journal Published Year Pages File Type
10843186 Protein Expression and Purification 2013 13 Pages PDF
Abstract
► The gene for rice bran lipase was cloned. ► RBL was expressed as a functional secretory protein in Pichia pastoris. ► The biochemical and kinetic properties of the expressed protein was similar to RBL. ► Expressed RBL showed a preference for short chain fatty acids. ► Catalytic efficiency correlates with the distance between Ser-OH to scissile ester bond.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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