Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10843186 | Protein Expression and Purification | 2013 | 13 Pages |
Abstract
⺠The gene for rice bran lipase was cloned. ⺠RBL was expressed as a functional secretory protein in Pichia pastoris. ⺠The biochemical and kinetic properties of the expressed protein was similar to RBL. ⺠Expressed RBL showed a preference for short chain fatty acids. ⺠Catalytic efficiency correlates with the distance between Ser-OH to scissile ester bond.
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Authors
K.R. Vijayakumar, Lalitha R. Gowda,