| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843187 | Protein Expression and Purification | 2013 | 5 Pages |
Abstract
⺠Recombinant SELA present a bound RNA compromising functional analysis. ⺠A simple expression system was developed in a tRNAsec free Escherichia coli strain. ⺠SELA molecular characterization shows it is equivalent to the native enzyme. ⺠Crosslinking experiments show that homodecamer formation is a sequential pathway.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Livia Regina Manzine, Alexandre Cassago, Marco Túlio Alves da Silva, Otavio Henrique Thiemann,