| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843191 | Protein Expression and Purification | 2013 | 7 Pages |
Abstract
⺠A heterodimeric AM receptor ECD complex was purified using novel folding methodology. ⺠The RAMP2 ECD was properly folded by co-expression with DsbC in E. coli trxB gor. ⺠Proper folding of an MBP-CLR ECD fusion protein was dependent on RAMP2 ECD. ⺠DsbC-assisted disulfide shuffling enhanced RAMP2-dependent CLR ECD folding in vitro. ⺠The recombinant MBP-CLR:RAMP2 ECD complex selectively bound AM.
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Authors
Heather E. Hill, Augen A. Pioszak,