Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10843193 | Protein Expression and Purification | 2013 | 7 Pages |
Abstract
⺠With RBS substitution, soluble DAAO expression was increased over 2-fold under T7 promoter. ⺠Constitutive expression system with Tac promoter and optimal RBS improved DAAO activity 20-fold. ⺠Expression rate optimization proved effective in the expression of other emzymes GCA and D-case.
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Authors
Jixian Hou, Yangqiu Liu, Qiang Li, Jichu Yang,