Article ID Journal Published Year Pages File Type
10843193 Protein Expression and Purification 2013 7 Pages PDF
Abstract
► With RBS substitution, soluble DAAO expression was increased over 2-fold under T7 promoter. ► Constitutive expression system with Tac promoter and optimal RBS improved DAAO activity 20-fold. ► Expression rate optimization proved effective in the expression of other emzymes GCA and D-case.
Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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