| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843199 | Protein Expression and Purification | 2013 | 8 Pages |
Abstract
⺠Protein O-mannosyltransferase 1 is the key enzyme involved in O-mannosylation. ⺠Multiple PMT genes from Pichia were inactivated and checked for reduction in O-mannosylation of insulin precursor. ⺠PMT1 gene inactivation resulted in â¼60% reduction in O-mannosylation of insulin precursor. ⺠The mannosylation site was identified by peptide mapping using the protease Glu-C and mass spectroscopy.
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Authors
Nagaraj Govindappa, Manjunatha Hanumanthappa, Krishna Venkatarangaiah, Komal Kanojia, Krishnamurthy Venkatesan, Amarnath Chatterjee, Mutyalasetty Kusumanchi, Nitesh Dave, Partha Hazra, Sanjay Tiwari, Kedarnath Sastry,