Article ID Journal Published Year Pages File Type
10843207 Protein Expression and Purification 2012 5 Pages PDF
Abstract
► Escherichia coli was cultivated at 6-10 °C to produce recombinant proteins. ► E. coli strains without cold adapted chaperones grew at the extremely low temperatures. ► The active soluble proteins were produced in large quantity under this condition. ► This strategy is effective for overcoming the insolubility of recombinant proteins.
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Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
Preview
Cultivation at 6-10 °C is an effective strategy to overcome the insolubility of recombinant proteins in Escherichia coli
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