Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10843210 | Protein Expression and Purification | 2012 | 8 Pages |
Abstract
⺠Nature and position of affinity tag is critical for mutant CBS folding and assembly. ⺠C-terminal 6xHis-tagged WT and R266K CBS has the highest activity among all constructs. ⺠The heme in R266K mutant is more susceptible to ligand switch than WT. ⺠The R266K mutation most likely destabilizes the thiolate ligand of the heme.
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Authors
Tomas Majtan, Jan P. Kraus,