Article ID Journal Published Year Pages File Type
10843210 Protein Expression and Purification 2012 8 Pages PDF
Abstract
► Nature and position of affinity tag is critical for mutant CBS folding and assembly. ► C-terminal 6xHis-tagged WT and R266K CBS has the highest activity among all constructs. ► The heme in R266K mutant is more susceptible to ligand switch than WT. ► The R266K mutation most likely destabilizes the thiolate ligand of the heme.
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