Article ID Journal Published Year Pages File Type
10843212 Protein Expression and Purification 2012 7 Pages PDF
Abstract
► We improved heterologous expression of a mammalian methyl-CpG binding domain. ► Temperature at induction had a dramatic effect on soluble yield. ► A single-step purification yielded 11.3 mg/L (174 μg/L for existing procedures). ► This improved yield enables development of epigenetic profiling assays.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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Functional heterologous expression and purification of a mammalian methyl-CpG binding domain in suitable yield for DNA methylation profiling assays
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