Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10843221 | Protein Expression and Purification | 2012 | 6 Pages |
Abstract
⺠The oligomeric c subunit ring was purified from the thermophilic FoF1-ATP synthase. ⺠A key in the purification was the introduction of a virtual His10-tag to the c-ring. ⺠The intactness of the isolated c-ring was confirmed by its homogeneity and activity. ⺠The isolated TFoc-rings were reconstituted into lipid membranes.
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Authors
Ikuko Yumen, Iku Iwasaki, Toshiharu Suzuki, Yasuto Todokoro, Kentaro Tanaka, Osamu Okada, Toshimichi Fujiwara, Masasuke Yoshida, Hideo Akutsu,