| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843228 | Protein Expression and Purification | 2012 | 7 Pages |
Abstract
⺠Protein production by auto-induction was higher than IPTG induction in a modified LB medium. ⺠Galactose mediated auto-induction was identified by medium analysis and spiking induction experiments. ⺠Escherichia coli BL21 cultures induced by galactose can saturate at considerably higher density than those induced by IPTG. ⺠Galactose was not consumed by E. coli BL21. ⺠Auto-induced fed-batch fermentation was demonstrated for effective protein production at 400-L scale.
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Authors
Jianlin Xu, Akhilesh Banerjee, Shih-Hsie Pan, Zheng Jian Li,