Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10843230 | Protein Expression and Purification | 2012 | 7 Pages |
Abstract
⺠Rec. glutaminase (GlsA) from Bacillus licheniformis was efficiently expressed in Escherichia coli. ⺠Microbial glutaminases are enzymes with potential in biotechnological industries. ⺠Rec. GlsA was 10ÃHis tagged and could be efficiently purified by one-step IMAC. ⺠Potential application for bioconversion of glutamine to glutamic acid was shown.
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Authors
Sornchai Sinsuwan, Jirawat Yongsawatdigul, Suchintana Chumseng, Montarop Yamabhai,