| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843232 | Protein Expression and Purification | 2012 | 5 Pages |
Abstract
⺠The His-AtCaN2 fusion protein was expressed and purified in Escherichia coli. ⺠The optimal temperature of His-AtCaN2 nuclease activity was 37 °C. ⺠His-AtCaN2 nuclease activity required a neutral or weakly-alkaline environment. ⺠His-AtCaN2 nuclease activity was stimulated by Ca2+ and Mg2+. ⺠His-AtCaN2 nuclease activity was inhibited by Zn2+ and high concentration of Mn2+.
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Authors
Kunyuan Guo, Shenkui Liu, Tetsuo Takano, Xinxin Zhang,