Article ID Journal Published Year Pages File Type
10843233 Protein Expression and Purification 2012 9 Pages PDF
Abstract
► We overexpress the human N-terminal domain of T1R3 (hT1R3-NTD) as inclusion bodies using Escherichia coli. ► Using a fractional factorial screen, we found conditions for the refolding of hT1R3-NTD. ► Biophysical approaches showed that hT1R3-NTD is functional. ► This strategy is a prerequisite for structural determination of NTD of other class C GPCRs.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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Recombinant expression, in vitro refolding, and biophysical characterization of the N-terminal domain of T1R3 taste receptor
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