| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843234 | Protein Expression and Purification | 2012 | 8 Pages |
Abstract
⺠A method for improved antibody fragment expression in Escherichia coli is proposed. ⺠Reformatting of scFvs into scAbs significantly enhances expression and screening performance. ⺠High precision kinetic analysis was performed using a 'customised' Biacore applications wizard. ⺠The best clone selected was incorporated into a sandwich-based immunoassay for PSA. ⺠Initial antibody format design significantly effects potential clone selection quality.
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Authors
C.J. Hayes, P. Leonard, R. O'Kennedy,