Article ID Journal Published Year Pages File Type
10843240 Protein Expression and Purification 2012 5 Pages PDF
Abstract
► We have produced recombinant procathepsin V in soluble form in Escherichia coli. ► The zymogen was activated autocatalytically at 30 °C. ► The protocol also proved successful for the production of other cysteine cathepsins.
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Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
Preview
A simple and efficient protocol for the production of recombinant cathepsin V and other cysteine cathepsins in soluble form in Escherichia coli
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