| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843244 | Protein Expression and Purification | 2012 | 6 Pages |
Abstract
⺠Cell-free protein synthesis based on E. coli is limited by mRNA secondary structures. ⺠RNA chaperones and helicases cannot increase yields in E. coli cell-free systems. ⺠A miRNA hybridizing with the 5ⲠUTR of mRNAs enhances translation. ⺠miRNAs might provide a general mechanism to increase yields in cell-free systems.
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Authors
Axel Freischmidt, Michael Liss, Ralf Wagner, Hans Robert Kalbitzer, Gudrun Horn,