Article ID Journal Published Year Pages File Type
10843247 Protein Expression and Purification 2012 10 Pages PDF
Abstract
► Eight fluorescent chimeras of human small heat shock proteins (sHsp) were obtained. ► Spectral properties of fluorescent proteins (FP) were not changed after fusing to sHsp. ► Fusing of fluorescent proteins did not affect oligomeric state of HspB6 and HspB8. ► HspB1 and HspB5 chimeras form oligomers containing less subunits than wild type sHsp. ► Cyan FP fused to small heat shock proteins enhanced their chaperone-like activity.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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