Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10843247 | Protein Expression and Purification | 2012 | 10 Pages |
Abstract
⺠Eight fluorescent chimeras of human small heat shock proteins (sHsp) were obtained. ⺠Spectral properties of fluorescent proteins (FP) were not changed after fusing to sHsp. ⺠Fusing of fluorescent proteins did not affect oligomeric state of HspB6 and HspB8. ⺠HspB1 and HspB5 chimeras form oligomers containing less subunits than wild type sHsp. ⺠Cyan FP fused to small heat shock proteins enhanced their chaperone-like activity.
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Authors
Petr N. Datskevich, Evgeny V. Mymrikov, Nikolai N. Sluchanko, Anton A. Shemetov, Maria V. Sudnitsyna, Nikolai B. Gusev,