| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843256 | Protein Expression and Purification | 2012 | 9 Pages |
Abstract
⺠We describe the production of a fungal carboxypeptidase in the cytosol of Escherichia coli. ⺠The zymogen is kept soluble by fusing it to E. coli maltose-binding protein. ⺠Co-expression of DsbC in the cytosol promotes the formation of disulfide bonds. ⺠The zymogen is activated by digestion with thermolysin. ⺠Active carboxypeptidase is purified by affinity and conventional chromatography.
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Authors
Brian P. Austin, David S. Waugh,