Article ID Journal Published Year Pages File Type
10843259 Protein Expression and Purification 2012 6 Pages PDF
Abstract
► Immobilized metal affinity chromatography was innovatively used to purify GSE-BL. ► Gradual maturation process of GSE-BL was revealed. ► The presence of pro-peptide could decrease specific activity of GSE-BL. ► GSE-BL is a relatively stable and specific endopeptidase.
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Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
Preview
Cloning, expression, purification, and characterization of a glutamate-specific endopeptidase from Bacillus licheniformis
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