| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843282 | Protein Expression and Purification | 2012 | 7 Pages |
Abstract
⺠APPTM has been successfully overexpressed as a fusion protein with MBP. ⺠Pure APPTM can be obtained by Ni-NTA chromatography, TEV protease cleavage and RP-HPLC. ⺠Isotopically labeled APPTM WT and an FAD mutant were purified for NMR studies. ⺠Excellent HSQC spectra were obtained in DPC and SDS micelles.
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Biochemistry
Authors
Wen Chen, Eric Gamache, Danielle Richardson, Zhenming Du, Chunyu Wang,