Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10843294 | Protein Expression and Purification | 2012 | 6 Pages |
Abstract
⺠(His)6-tagged human arginase I was expressed in the yeast Saccharomyces cerevisiae. ⺠The recombinant arginase was purified in one step by Nickel-affinity chromatography. ⺠The purified enzyme exhibits a specific activity up to 1600 μmol minâ1 mgâ1 protein.
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Authors
Andriy E. Zakalskiy, Oksana M. Zakalska, Yuriy A. Rzhepetskyy, Natalia Potocka, Oleh V. Stasyk, Daniel Horak, Mykhailo V. Gonchar,