| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843295 | Protein Expression and Purification | 2012 | 7 Pages |
Abstract
⺠Functional human GNA1 and GNA1-sGFP were highly expressed by cell-free system. ⺠Inhibition assay with glucose-6-phosphate was performed and IC50 was calculated. ⺠Binding sites of glucose-6-phosphate to GNA1 were identified by simulated annealing. ⺠This strategy dramatically reduces the timeline for GNA1 analysis and screening.
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Authors
Yi Ma, Umesh Ghoshdastider, Jufang Wang, Wei Ye, Volker Dötsch, Slawomir Filipek, Frank Bernhard, Xiaoning Wang,