| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843298 | Protein Expression and Purification | 2012 | 8 Pages |
Abstract
⺠Inclusion body proteins can be solubilized using 6 M n-propanol solution along with 2 M urea. ⺠Solubilization using n-propanol results in improved recovery of bioactive human growth hormone. ⺠Propanol promotes non-denaturing solubilization of inclusion body proteins. ⺠Propanol based solubilization buffer is milder than 8 M urea or 6 M guanidine hydrochloride.
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Authors
Surinder M. Singh, Aparna Sharma, Arun K. Upadhyay, Anupam Singh, Lalit C. Garg, Amulya K. Panda,