| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843302 | Protein Expression and Purification | 2012 | 7 Pages |
Abstract
⺠We expressed the ToMV replication proteins that contain the helicase domain. ⺠We determined the stable helicase fragment. ⺠Its N-terminal part was necessary for NTPase activity and stability. ⺠We developed an efficient expression and purification procedures for the fragment. ⺠The stable fragment has nucleoside 5â²-triphosphatase activity.
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Authors
Hongyu Xiang, Kazuhiro Ishibashi, Masaki Nishikiori, Mauren C. Jaudal, Masayuki Ishikawa, Etsuko Katoh,