Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10843308 | Protein Expression and Purification | 2012 | 10 Pages |
Abstract
⺠We report cloning, expression, and purification of CC, LZ, and CCLZ domains of MBS. ⺠Applied methods result in high level protein expression of 15N and 13C labeled MBS polypeptides. ⺠SEC, CD, and 1H-15N HSQC demonstrate that expressed MBS CC domain is folded and exists as a dimer. ⺠Expressed MBS LZ domain is unfolded and non-stable at physiological pH. ⺠Expressed MBS LZ domain adopts folded and stable conformation at acidic pH 4.5.
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Authors
Alok K. Sharma, Paramvir Sawhney, Gonen Memisoglu, Alan C. Rigby,