Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10843316 | Protein Expression and Purification | 2011 | 6 Pages |
Abstract
The majority of current high-throughput protein purification protocols include rate-limiting centrifugation steps. A column and nozzle assembly was developed that can be used in-line with microfluidization for the purification of bacterially-overexpressed, His-tagged proteins directly from bacterial cultures. Yields and purity are comparable with standard protocols. This large-scale protein purification protocol is easy to use and widely-applicable.
Keywords
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Biochemistry
Authors
Denis Alenkin, Laila Yermekbayeva, Shariq Mujib, Anders Vesterberg, Elena Newman, Ken Yamazaki, Doug Cossar, Sirano Dhe-Paganon,