| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843319 | Protein Expression and Purification | 2011 | 8 Pages |
Abstract
⺠Xylulose kinase (XKS1) is a key enzyme for xylose utilization in Saccharomyces cerevisiae. ⺠XKS1 was recombinantly produced in E. coli, and native and tagged forms of the enzyme were isolated. ⺠XKS1 was highly unstable, losing its activity rapidly during purification or storage. ⺠Isolated XKS1 was shown by MS to be structurally intact. ⺠XKS1 harboring a C-terminal Strep-tag II was purified with retention of activity and characterized.
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Authors
Simone L. Pival, Ruth Birner-Gruenberger, Corinna Krump, Bernd Nidetzky,