| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843327 | Protein Expression and Purification | 2011 | 8 Pages |
Abstract
⺠We have purified enzymatically active alphavirus capping enzyme, nsP1. ⺠Purified Sindbis virus (SINV) nsP1 possess the methyltransferase (MTase) and guanylyltransferase (GTase) activities. ⺠This indicates that alphavirus nsP1 does not require membrane association for its enzymatic function. ⺠SINV nsP1 GTase activity is metal-ion dependent, whereas MTase does not require a metal ion. ⺠CD analysis shows that the nsP1 has a mixed α/β structure consisting of 32.8% α-helix, 19.5% β-sheet, 19% β-turn and 32.5% random coil.
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Authors
Shailly Tomar, Manju Narwal, Etti Harms, Janet L. Smith, Richard J. Kuhn,