Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10843414 | Protein Expression and Purification | 2008 | 7 Pages |
Abstract
The whole encoding sequence for Yersinia pestis LcrV antigen was cloned into pET-32a(+) and expressed in Escherichia coli BL21 (DE3). The LcrV was high level expressed in the E. coli cytoplasm in a completely soluble form. Recombinant LcrV could be purified from the supernatant of the bacteria lysate after chromatography using a combination of Phenyl-Sepharose F F, DEAE-Sepharose F F and Hiload⢠Superdex⢠75. The final yield of approximately 3 g of purified rLcrV from 42 L bioreactor containing 25 L LB medium was obtained. High-titer IgG directed against rLcrV was detected positive after immunization on the BALB/c mice. The results presented here exhibit the ability to generate multi-gram quantities of non-tagged rLcrV from E. coli that can be used for the development of vaccine for preventing plague.
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Authors
Jianmin li, Bing Li, Guanlin Li, Jun Ren, Jinlong Zhang, Chun'e Xu, Xiuxu Yang, Shuling Liu, Ling Fu, Wei Chen,