| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10843463 | Protein Expression and Purification | 2007 | 6 Pages |
Abstract
Production of recombinant proteins at low temperatures is one strategy to prevent formation of protein aggregates and the use of an expensive inducer such as IPTG. We report on the construction of two expression vectors both containing the cold-inducible des promoter of Bacillus subtilis, where one allows intra- and the other extracellular synthesis of recombinant proteins. Production of recombinant proteins started within the first 30 min after temperature downshock to 25 °C and continued for about 5 h.
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Authors
Ai Thi Thuy Le, Wolfgang Schumann,